an unscheduled DNA synthesis assay, and a micronucleus assay in mice

and  activity in the liver was assessed histochemically and by quantitative fluorimetric assay..
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and by quantitative fluorimetric assay


Our findings suggest that and inhibit synthesis and secretion of proteins by human airway by suppressing -mediated of signaling molecules such as RhoA..

In a mutagenic assay in human diploid fibroblasts styrene oxide failed to elicit an increase in unscheduled DNA synthesis (McGregor, 1983).
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OECD Guidelines for the Testing of Chemicals - Wikipedia

As mentioned above, one motivation for developing this AOP is to address the current needs for improved testing in the field of germ cell mutagenesis. The recent release of the transgenic rodent mutation assay (TG 488) addresses this gap and is expected to be used more frequently in the future. In addition, new approaches that apply advanced sequencing technologies are expected to more fully assess the impact of environmental agents on induced heritable mutations and population health in the future. Finally, the lack of effective in high throughput mutation detection strategies, especially for germ cells, is a critical gap in this field. It is hoped that this AOP will provide a motivation to promote further research to address this gap, and serve as a basis to develop improved modelling approaches to predict whether a chemical agent is able to penetrate the testes and germ cells, alkylate DNA and cause heritable mutations.

assay with or without activation and unscheduled DNA synthesis assay in rat ..
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coli assays (negative); chromosome aberration assays in V79 Chinese hamster lung fibroblasts, with and without metabolic activation (positive); gene mutation assay in the HGPRT-test with V79 Chinese hamster lung fibroblasts (negative); unscheduled DNA synthesis in human A549 cells (negative); and BALB/c3T3 cell transformation assay (negative).

Both assays were negative; an unscheduled DNA synthesis assay in rat primary hepatocytes in vitro.
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