30/11/2017 · Trehalose – The Inse..
Heterologous expression of Escherichia coli ppsA (phosphoenolpyruvate synthetase) and galU (UDP-glucose pyrophosphorylase) genes in Corynebacterium glutamicum, and its impact on trehalose synthesis.
Insights on the evolution of trehalose biosynthesis | …
Osmoregulation in the model organism Escherichia coli: genes governing the synthesis of glycine betaine and trehalose and their use in metabolic engineering of stress tolerance.
TRET mRNA levels decreased in JHAMT and Met RNAi insects when compared to their levels in control insects in the alimentary canal but not in the fat body or head of starved insects, suggesting that JH regulates the expression of this gene in the alimentary canal (). Moreover, topical application of JH III induced the expression of the gene coding for TRET in the alimentary canal but not in the fat body or head (). Similarly, the mRNA levels of trehalase in the fat body decreased in JHAMT, Met, and ILP2 RNAi insects (). A decrease in mRNA levels of trehalase was observed in the alimentary canal isolated from JHAMT and Met RNAi beetles (). Similarly, head tissue dissected from JHAMT, ILP2, and Met RNAi insects showed a decrease in trehalase mRNA levels (). Topical application of JH III induced the gene coding for trehalase in the fat body but not in the alimentary canal or head (). Injection of insulin into starved males on day 5 induced trehalase gene expression by 2.2 and 1.9-fold in the fat body and head respectively when compared to the levels in the same tissues dissected from control beetles (). These data suggest that both JH and insulin regulate expression of the gene coding for trehalase and JH but not insulin regulates expression of the gene coding for TRET.