Insist on the Transcriptor First Strand cDNA Synthesis Kit ..
This is the first time that I will be peforming qRT-PCR.
I will be carrying out a two-step qRT-PCR, which involve the first step of first strand cDNA synthesis.
I come across two kit which is the "Transcriptor First Strand cDNA Synthesis Kit" and "1st Strand cDNA Synthesis Kit for RT-PCR
(AMV)". Both o the kit are from Roche.
Can anyone help me with the choice between these two kits?
Transcriptor First Strand cDNA Synthesis Kit -
1st Strand cDNA Synthesis Kit for RT-PCR (AMV) -
transcriptor first strand cdna synthesis kit
In order to acquire a total score for each kit, the sum of the success rate, ABL1 and linearity scores was observed. Using this strategy, all three parameters were taken into consideration for the evaluation of the different cDNA synthesis kits. Based on our findings, the kit with the highest acquired score was the SuperScript® III Reverse Transcriptase, by Invitrogen, achieving an overall score of 18, followed by the First Strand cDNA Synthesis Kit for RT-PCR, by Roche, with an overall score of 14 (Table ).
At first, a success rate analysis was performed, where the reverse transcription enzyme was considered successful if it amplified the BCR from the cDNA. The success rate was calculated based on the number of wells that were successfully amplified divided by the total well number for BCR amplification, with the enzyme with the highest success rate achieving the highest score (with the highest score being 6 and the lowest 1). All kits detected 0.32% BCR-ABL1 copy numbers. Using the SuperScript® III Reverse Transcriptase kit the BCR was successfully amplified from the cDNA at all levels, achieving a score of 6 (18/18). QuantiTect® Reverse Transcription, First Strand cDNA Synthesis kit for RT-PCR and the Transcriptor First Strand cDNA Synthesis kit achieved 15 successful reads at the 0.0032% level, attaining a score of 5 (15/18). AffinityScript Multiple Temperature cDNA Synthesis kit achieved 13 successful reads at the 0.0032% level, attaining a score of 2 (13/18) and the iScript™ Select cDNA Synthesis kit recorded 5 successful reads at the 0.032% level and one successful read at the 0.0032% level (12/18), attaining the lowest score of 1 (Additional file : Table S1).