Bacterial Cell Wall Synthesis Inhibitors | Clinical Gate

 html5 version of animation for iPad illustrating how penicillins inhibit peptidoglycan synthesis.

Chapter 46 Bacterial Cell Wall Synthesis Inhibitors

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Single molecule detection of a protein involved in peptidoglycan synthesis

Novel inhibitors of bacterial cell wall synthesis

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Peptidoglycan monomers - consisting of the sugars NAM and NAG with a pentapeptide coming off the NAM - are synthesized in the cytosol of the bacterium. These monomers are then transported across the cytoplasmic membrane and inserted into the growing peptidoglycan chain by membrane transporters called bactoprenols.


Cell Wall Biosynthesis - eLS: Essential for Life Science

Beta-lactams target the synthesis of the main component of the bacterial cell wall, peptidoglycan. The synthesis of peptidoglycan is the target of various classes of antibiotics, as it is an essential component virtually all bacteria and it is absent from eukaryotic cells. We want to understand the mechanism of action of existing and new antibacterial compounds, as well as the mechanisms of resistance developed by bacteria.

Bacterial Cell Wall Biosynthesis

During normal bacterial growth, bacterial enzymes called autolysins put breaks in the peptidoglycan in order to allow for insertion of new peptidoglycan monomers - consisting of NAG, NAM, and a pentapeptide. These monomers are then attached to the growing end of the bacterial cell wall with transglycosidase enzymes. Finally, transpeptidase enzymes (also called penicillin-binding proteins) form a peptide bridge that cross-links the peptides coming off of each NAM. These links connect each row of sugars with its adjacent rows and each layer of peptidoglycan with its adjacent layers. This is what gives peptidoglycan its strength.

Simulations of Bacterial Cell Wall Synthesis - YouTube

Scheible WR and Pauly M (2004) Glycosyltransferases and cell wall biosynthesis: novel players and insights. Current Opinion in Plant Biology 7: 285–295.

total synthesis of the bacterial cell wall precursor and ..

During normal bacterial growth, bacterial enzymes called autolysins put breaks in the peptidoglycan in order to allow for insertion of new peptidoglycan monomers - consisting of NAG, NAM, and a pentapeptide. These monomers are then attached to the growing end of the bacterial cell wall with transglycosidase enzymes. Finally, transpeptidase enzymes (also called penicillin-binding proteins) form a peptide bridge that cross-links the peptides coming off of each NAM. These links connect each row of sugars with its adjacent rows and each layer of peptidoglycan with its adjacent layers. This is what gives peptidoglycan its strength.

The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic ..

Penicillin Binding Protein 4 (PBP4) was tagged with Photoactivable mCherry and expressed Staphyloccus aureus. Live cells were observed by Photoactivated Localization Microscopy (PALM). Scale bar 500 nm.