Peptide Synthesizer, Automated Peptide Synthesizer
The addition of HOBt to the piperidine solution helps to reduce the formation of aspartimide. Another approach is to introduce the residue preceeding Asp as the Fmoc(Hmb) protected amino acid .
Solid-phase oligonucleotide synthesis - ATDBio
When the color tests keep revealing the presence of unreacted amino functions after recoupling, it is necessary to cap these to avoid the formation of deletion sequences. The capping will yield a truncated sequence (shortened pep- tide) but the truncated sequences differ generally considerably from the final peptide and can be readily separated.
After the cleavage the sample is analyzed by HPLC as Fmoc protected and free amino sequences are usually well separated in a standard HPLC gradient.
ChemPep - Peptide Synthesis, Peptide Array, HATU, …
Fmoc amino acid chlorides can also be used but their applicability is more limited ; also acid-sensitive groups may not be present when treating Fmoc amino acids with oxalyl chloride or thionyl chloride to obtain the acid chlorides [34,35].
ChemPep: One stop for peptide synthesis
Also small scale manual SPPS as well as multiple peptide synthesis and the synthesis of pep- tide libraries can be performed very rapidly and conveniently with preformed active esters. Fmoc-AA-ONp and Fmoc-AA-OSu have found only restricted application in SPPS.
Resins for Solid Phase Peptide synthesis Unsubstituted Resins
Thus, amino and hydroxy groups have been attached onto classical resins to give straightforward access to the solid-phase synthesis of alcohols, carbamates, and cyclic peptides with an evident pharmaceutical interest.">
solid phase peptide synthesis | resin manufacturers …
Thus, amino and hydroxy groups have been attached onto classical resins to give straightforward access to the solid-phase synthesis of alcohols, carbamates, and cyclic peptides with an evident pharmaceutical interest.
ChemMatrix ® Resins Solid-Phase Peptide ..
The same is true for the activation of Fmoc-His(Trt)-OH since racemization catalyzed by the nitrogen of the imidazole ring may occur. For the coupling of especially bulky amino acids such as Aib, Tic, ... or in the case of recognized difficult coupling we recommend the replacement of HOBt by HOAt, or the use of other activating agents.
Resins for Solid Phase Peptide and Organic Synthesis
Repeated incomplete deprotection of the αamino function as well as difficulties in obtaining a complete coupling reaction are some of the problems caused by the on resin aggregation of the peptide chain.