Proline biosynthesis in Escherichia coli

Delauney AJ, Verma DPS 1993 Proline biosynthesis and osmoregulation in plants.

Regulation of Proline Biosynthesis in ..

Hare and Cress (1997) argue that the different subcellular localizations of proline biosynthesis (cytoplasm) and oxidation (mitochondrion), the NADPH cofactor preference of the biosynthetic enzymes, and NADH cofactor preference for the proline oxidation pathway, would enable proline biosynthesis to enhance activity of the cytoplasmic oxidative pentose phosphate pathway, and provide a mechanism of interconversion of the phosphorylated and non-phosphorylated pools of pyridine nucleotide cofactors.

Delauney AJ, Verma DPS 1993 Proline biosynthesis and osmoregulation in plants.

Proline synthesis is implicated as a mechanism ..

Lodata RF, Smith RJ, Valle D, Phang JM, Aoki TT 1981 Regulation of proline biosynthesis: the inhibition of pyrroline-5-carboxylate synthase activity by ornithine.

Hong-qi Z, Croes AF, Linskens HF 1982 Protein synthesis in germinating pollen of : role of proline.

Savoure A, Hua X-J, Bertauche N, Van Montagu M, Verbruggen N 1997 Abscisic acid-independent and abscisic acid-dependent regulation of proline biosynthesis following cold and osmotic stresses in .

Thompson JF 1980 Arginine synthesis, proline synthesis, and related processes.


MetaCyc L-ornithine degradation I (L-proline biosynthesis)

The bicyclic β-lactam/2-pyrrolidine precursor to all carbapenem antibiotics is biosynthesized by attachment of a carboxymethylene unit to C5 of L-proline followed by β-lactam ring closure. Carbapenem synthase (CarC), an Fe(II) and 2-(oxo)glutarate (Fe/2OG)-dependent oxygenase, then inverts the C5 configuration. Here we report the structure of CarC in complex with its substrate and biophysical dissection of its reaction to reveal the stereoinversion mechanism. An Fe(IV)-oxo intermediate abstracts the hydrogen (H•) from C5, and tyrosine 165, a residue not visualized in the published structures of CarC lacking bound substrate, donates H• to the opposite face of the resultant radical. The reaction oxidizes the Fe(II) cofactor to Fe(III), limiting wild-type CarC to one turnover, but substitution of the H•-donating tyrosine disables stereoinversion and confers to CarC the capacity for catalytic substrate oxidation.

Effect of Nacl and Boron Toxicity on Proline Biosynthesis …

Kishor PBK, Hong Z, Miao G-H, Hu C-AA, Verma DPS 1995 Overexpression of delta-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants.

Regulation of proline biosynthesis, degradation, …

Hu CA, Delauney AJ, Verma DP 1992 A bifunctional enzyme (delta-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants.

The regulation of proline biosynthesis can be dependent on the ..

AB - The bicyclic β-lactam/2-pyrrolidine precursor to all carbapenem antibiotics is biosynthesized by attachment of a carboxymethylene unit to C5 of L-proline followed by β-lactam ring closure. Carbapenem synthase (CarC), an Fe(II) and 2-(oxo)glutarate (Fe/2OG)-dependent oxygenase, then inverts the C5 configuration. Here we report the structure of CarC in complex with its substrate and biophysical dissection of its reaction to reveal the stereoinversion mechanism. An Fe(IV)-oxo intermediate abstracts the hydrogen (H•) from C5, and tyrosine 165, a residue not visualized in the published structures of CarC lacking bound substrate, donates H• to the opposite face of the resultant radical. The reaction oxidizes the Fe(II) cofactor to Fe(III), limiting wild-type CarC to one turnover, but substitution of the H•-donating tyrosine disables stereoinversion and confers to CarC the capacity for catalytic substrate oxidation.

which leads to proline biosynthesis, ..

Aral B, Schlenzig J-S, Liu G, Kamoun P 1996 Database cloning of human delta-pyrroline-5-carboxylate synthetase (P5CS) cDNA: a bifunctional enzyme catalyzing the first 2 steps in proline biosynthesis.