Click Chemistry -- Publications - Scripps Research Institute
To make the solid support material suitable for oligonucleotidesynthesis, non-nucleosidic linkers or nucleoside succinates arecovalently attached to the reactive amino groups in AminopropylCPG, LCAA CPG, or Aminomethyl MPPS. The remaining unreacted aminogroups are capped with . Typically, threeconceptually different groups of solid supports are used.
Designing PCR primers and probes
Why should you consider Novatia and ESI/LC/MS for your oligonucleotide characterization? Novatia uses electrospray ionization liquid chromatography mass spectrometry (ESI/LC/MS) to characterize oligonucleotides. We can perform these analyses in a high-throughput mode to confirm the desired products from oligo synthesis reactions or in a more detailed mode using high resolution chromatographic separation. Most laboratories uses MALDI-ToF and/or gel electrophoresis to characterize oligos. Novatia has developed the , which provides a number of advantages relative to MALDI-ToF and other traditional methods for oligo characterization. Our methods are fast, cost-effective, and informative!
Oligonucleotide synthesis is carried out by a stepwise additionof nucleotide residues to the 5'-terminus of the growing chainuntil the desired sequence is assembled. Each addition is referredto as a synthetic cycle (Scheme 6) and consists of four chemicalreactions: