Peptide Chemistry Peptide Synthesis and Purification
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Chymotrypsin - Worthington Enzyme Manual
Synthesis: Peptides were either synthesized manually or with peptide synthesizers according to Fmoc-strategy, purified by RP-HPLC and analyzed with MS.
Solid-phase peptide synthesis (SPPS), pioneered by Merrifield, resulted in a paradigm shift within the peptide synthesis community. It is now the accepted method for creating peptides and proteins in the lab in a synthetic manner. SPPS allows the synthesis of natural peptides which are difficult to express in bacteria, the incorporation of unnatural amino acids, peptide/protein backbone modification, and the synthesis of D-proteins, which consist of D-amino acids.