beta-Lactams and other Cell Wall Inhibitors - 4 cards

Erythropoiesis: testosterone promotes red blood cell formation and protects against anemia.

Plant Development-Introduction - Plant Cell Biology …

In all animal cell lines tested to date, even at five times the working concentration, no apparent adverse effect on cellular metabolism is observed.

Replacing testosterone in males with hypogonadism results in increases in red blood cell mass.

Cell Wall Synthesis Inhibitors | Beta Lactamase | Penicillin

Numerous studies have shown that imiquimod also inhibits the growth of new blood vessels thanks to its antiangiogenic properties. It induces an increase in IL-10 and IL-12 levels, which inhibit angiogenesis, reduce cell production of proangiogenic factors (such as fibroblast growth factor and IL-8), inhibit vascular motility, and induce endothelial apoptosis. There is also evidence that imiquimod induces keratinocyte apoptosis, thereby favoring cytochrome C release and caspase 3 activation.

Abbreviations: AK, actinic keratosis; BCC, basal cell carcinoma; sBCC, superficial basal cell carcinoma; SCC, squamous cell carcinoma.

Mechanism of action of 5-fluorouracil (5-FU), which binds to and inhibits the enzyme thymidylate synthase (TS), thereby reducing DNA synthesis and cell proliferation and inducing cell death.

The lack of response to diclofenac in SCC cells appears to be independent of pathways that modulate apoptosis through COX-2 in SCC cells.


Inhibition of cell wall synthesis by sulfonamides and trimethoprim

Plasmocin™ is used to cure cell lines infected by mycoplasma and related cell wall-less bacteria.
Plasmocin™ can also be used as a routine addition in liquid media to prevent mycoplasma and more generally bacterial contamination in small and large animal cell cultures.

Chapter 6 inhibitors of cell wall synthesis - SlideShare

In contrast to other anti-mycoplasma compounds, Plasmocin™ is active on both free mycoplasmas and intracellular forms. This advantage is conferred by one component of Plasmocin™ which is actively transported into mammalian cells. It ensures that following treatment with Plasmocin™ a cell culture is not reinfected by mycoplasmas released from intracellular compartments of infected cells.

possess a cell wall composed ..

Plasmocin™ is also active at low concentrations on a broad range of Gram positive and Gram negative bacteria that are otherwise resistant to the mixture of streptomycin and penicillin, and exhibits no toxicity in eukaryotic cells.

Antibiotics That Inhibit Cell Wall Synthesis - …

Many cell lines infected by mycoplasmas have been successfully treated with Plasmocin™, including embryonic stem cells, hybridomas and retrovirus packaging cells.

What are the inhibitors of cell wall synthesis

1. Uphoff CC et al., 2012. Treatment of mycoplasma contamination in cell cultures with Plasmocin. J Biomed Biotechnol. 2012:267678.
2. Rongvaux A et al., 2014. Development and function of human innate immune cells in a humanized mouse model. Nat Biotechnol. 32(4):364-72.
3. Baronti C. et al., 2013. Mycoplasma removal: simple curative methods for viral supernatants. J Virol Methods. 187(2):234-7
4. Deng F. et al. 2012. Generation of induced pluripotent stem cells from human Tenon's capsule fibroblasts. Mol Vis. 18:2871-81
5. Romorini L. et al, 2013. Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth. PLoS One. 8(7):e70267