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The functional categories with the largest number of differently expressed unigenes are the categories 'Metabolism' and 'Transport' (Figure ). Among these are four unigenes which we had isolated via the SSH approach (e.g. glycogen phosphorylase, UDP-glucose-4-epimerase, formate dehydrogenase; Table ). The high fold change cut-off used for microarray analysis as well as the lower sensitivity of microarrays compared to SSH probably limited the amount of overlapping hits. Nine unigenes predicted to encode polysaccharide-acting enzymes were up-regulated when S. rolfsii was cultivated in EPSmax13 medium (Table ), thus representing potential candidate genes involved in scleroglucan elongation and branching. Moreover, many up-regulated unigenes fall into the group of ergosterol and sphingolipid metabolic proteins (Table ). Finally, various unigenes assigned to transporters (ions, amino acids, peptides, lipids) and oxidoreductases (e.g. aryl-alcohol dehydrogenases) displayed altered expression under EPSmax13 conditions (Additional file ). These transcriptional changes could imply that scleroglucan synthesis might be coupled to the cellular ion homeostasis machinery. Such a scenario would be in agreement with the overall concept that microbial EPS production is also an adaptive response towards environmental salt and osmotic stress [-].