First cycle, double-stranded cDNA clean-up by ethanol precipitation.

Superscript III 1st Strand Synthesis Kit (Invitrogen, Cat .SMARTer™ Clontech Laboratories, Inc.

Second Cycle, first strand cDNA synthesis

Hi,

This is the first time that I will be peforming qRT-PCR.

I will be carrying out a two-step qRT-PCR, which involve the first step of first strand cDNA synthesis.

I come across two kit which is the "Transcriptor First Strand cDNA Synthesis Kit" and "1st Strand cDNA Synthesis Kit for RT-PCR
(AMV)". Both o the kit are from Roche.

Can anyone help me with the choice between these two kits?

Thanks.

Reference:
Transcriptor First Strand cDNA Synthesis Kit -
1st Strand cDNA Synthesis Kit for RT-PCR (AMV) -

amplification was performed without prior first strand cDNA synthesis

Www.roche-applied-science.com for general laboratory use. not for use in diagnostic procedures. for in vitro use only. transcriptor first strand cdna synthesis kit

cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl.