glycogen synthesis and degradation.
We report the first total synthesis of a lipoglycopeptide member of the arylomycin family of natural products, and we demonstrate that it effectively penetrates Gram-negative bacteria and is limited by the same resistance mechanism as other members of the arylomycin class of antibiotics (i.e. mutation in the target protein signal peptidase (SPase)). Unlike the other arylomycins, the lipoglycopeptides are glycosylated, and the structural data reveal that this sugar is most solvent exposed when in complex with SPase.
Biological degradation of plastics: A comprehensive …
As one of the attractive applications of PAA hydrolases, we report here the enzyme-catalyzed synthesis of poly(α-ethyl β-aspartate), which is composed of only β-linkages and belongs to β-peptides, using the unique substrate specificity of the enzyme from Pedobacter sp.
Systematic inorganic and organometallic chemistry of the main group elements, emphasizing structure, bonding and reactivity in inorganic and organometallic compounds. Selected experiments in inorganic and organometallic synthesis; use of modern structural methods for determination of composition, structure and bonding.
Cellulose Chemistry and Technology
Pyridyl nucleoside analogues are synthesized and characterized (see structures). An α-glycosidic nitrogen atom provides an H-bond acceptor that does not significantly facilitate pairing with natural nucleobases. However, it forms minor-groove H-bonds with water molecules and DNA polymerases that optimize the stability and replication, respectively, of the unnatural base pair.
Cellulose Chemistry and Tehnology ..
Introduction to aromatic molecules and electrophilic aromatic substitution reactions. Chemistry of natural products, their origin and biological significance. Topics include concepts of carbonyl and carbohydrate chemistry, DNA and an introduction to amino acids and proteins. Selected experiments in organic synthesis, and characterization and analysis.
catalyzed functionalization of medium-chain ..
We report the synthesis and analysis of the ribo- and deoxyribo-variants, (d)5SICS and (d)MMO2, modified with free or protected propargylamine linkers that allow for the site-specific modification of DNA or RNA during or after enzymatic synthesis. We also synthesized and evaluated the α-phosphorothioate variant of d5SICSTP, which provides a route to backbone thiolation and an additional strategy for the post-amplification site-specific labeling of DNA.
Detection of enzyme-catalyzed polysaccharide synthesis …
Our programs stress chemical principles and practice through research in organic, inorganic, analytical, physical and theoretical chemistry, with a strong emphasis on the interdisciplinary fields of bio-inorganic and bio-organic chemistry and chemical biophysics. Research interests of the department include development of new chiral ligands, organometallic reagents, and methods for asymmetric synthesis, green chemistry including bacterial dioxygenase-mediated degradation of aromatics, new approaches in natural product synthesis emphasizing morphine alkaloids, design and synthesis of fluorinated inhalation anesthetic agents and unnatural oligo-saccharide conjugates, ligand design and crystal engineering aimed at self-assembly of novel molecule-based materials, organic electrochemistry, energetics of photosynthesis studied by time-resolved electron spin resonance of short-lived paramagnetic intermediates, nuclear magnetic resonance spectroscopy, organometallic mass spectrometry, quantum and computational chemistry, pesticide and trace element analysis, protein affinity labelling and protein-lipid interactions, and structure-activity correlations of anticancer pharmaceuticals.
02/12/2009 · Biocatalysis and Biotransformation ..
Use of organometallic reagents for organic transformations. Metalation and trans-metalation using organolithium, magnesium, cerium and zinc reagents; stable precursors and reactive intermediates in transition metal catalyzed organic reactions employing palladium, nickel, ruthenium, and other metals. Topics from recent literature involving applications to organic synthesis, emphasizing stereoselectivity.