Mechanisms of Protein Synthesis by the Ribosome

Elongation of Protein Synthesis: Structural Basis of the Process of Decoding [Video file].

Elongation cycle of protein synthesis


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Bunai F, Ando K, Ueno H, Numata O (2006) eukaryotic translation elongation factor 1A (eEF1A) bundles filamentous actin through dimmer formation. Journal of Biochemistry 140, 393–399.

Protein Synthesis(Translation) | Simplified Biology


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Blumenthal T, Landers TA, Weber K (1972) Bacteriophage Qβ replicase contains the protein biosynthesis elongation factors EF-Tu and EF-Ts. Proceedings of the National Academy of Sciences of the United States of America 69, 1313–1317.


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Caldas TD, Yaagoubi AE, Richarme G (1998) Chaperone properties of bacterial elongation factor. Journal of Biological Chemistry 273, 11478–11482.


The Elongation Reactions in Protein Synthesis - …

The best known and studied bacterial toxin is the diphtheria toxin,produced by . Diphtheria toxin is abacterialexotoxin of the A/B prototype. It is produced as single polypeptidechainwith a molecular weight of 60,000 daltons. The function of the proteinis distinguishable into two parts: subunit A, with a m.w. of 21,000daltons,contains the enzymatic activity for inhibition of elongation factor-2involvedin host protein synthesis; subunit B, with a m.w. of 39,000 daltons, isresponsible for binding to the membrane of a susceptible host cell. TheB subunit possesses a region T (translocation) domain which insertsinto the endosome membrane thus securing the release of the enzymaticcomponent into the cytoplasm.

TRANSLATION INITIATION (Protein Synthesis) - what …


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Hotokezaka Y, Többen U, Hotokezaka H, van Leyen K, Beatrix B, Smith DH, Nakamura T, Wiedmann M (2002) Interaction of the eukaryotic elongation factor 1A with newly synthesized polypeptides. Journal of Biological Chemistry 277, 18545–18551.

Protein Synthesis Steps - Protein Synthesis

Each factor plays a particular role in translation initiation, acting together to form 30S initiation complexes that can proceed toward elongation. The precise function of IF1 is not known, although it does stimulate the binding and activities of IF2 and IF3. It binds to 30S subunits directly, and in combination with the other factors promotes the formation of 30S initiation complexes. Once the 30S complex is formed, IF1 is ejected along with IF3. To date there is no conclusive evidence that the IFs affect the Shine-Dalgarno interaction between the mRNA and rRNA.

Protein Synthesis -Translation and Regulation

(Academic Press: New York)

Li ZY, Chen SY (1999) Inducible expression of translation elongation factor 1A gene in rice seedlings in response to environmental stresses. Acta Botanica Sinica 41, 800–806.

Protein Synthesis - Translation Flashcards | Quizlet

Better results will be obtained by repeating a coupling with fresh reagents (and changing coupling parameters if a low conversion was obtained) rather than by prolonging the reaction. Generally, coupling protocols may be changed in the course of a synthesis, especially when optimizing an SPPS.