Double-Stranded cDNA Synthesis Kit - ZAGENO

RNA interference – gene silencing by double-stranded RNA will be available on

Synthesis of simian rotavirus SA11 double-stranded RNA …

InvivoGen also provides poly(I:C) in biotin and fluorescently-labeled forms, in VacciGradeTM form (certified sterile and endotoxin-free per LAL validation), plus polyadenylic–polyuridylic acid (usually abbreviated poly(A:U)), a synthetic dsRNA analog that signals exclusively through TLR3.

T1 - Synthesis and characterization of small circular double-stranded RNAs

Synthesis of double-stranded RNA by poliovirus …

It was only until recently that RNAi was found to work in mammalian systems.

Small interference RNA (siRNA) is 19-22 nt double-stranded RNA that works by cleaving and destroying its cognate RNA.

16/08/2016 · Synthesis of bacteriophage phi6 double stranded ribonucleic acid

Concentrations of double-stranded RNA above about 0.1 μg/ml inhibit translation of encephalo-myocarditis viral RNA and mouse globin messenger RNA in extracts of Krebs II ascites cells. Protein synthesis initially proceeds at the control rate, then abruptly shuts off in a manner similar to that observed in reticulocyte lysates [Hunt, T. & Ehrenfeld, E. (1971) Nature New Biol. 230, 91-94]. Substantially higher concentrations of double-stranded RNA are required to give this effect in ascites extracts.

Semiconservative Synthesis of Single-Stranded RNA by Bacteriophage φ6 RNA …


Double-stranded RNA-binding Proteins and the Control …

AB - Double-stranded RNA dependent protein kinase (PKR) is a host defense enzyme whose expression is up-regulated in response to interferons (IFNs) and during viral infections. Increased levels of PKR can result in its activation, which, in turn, inhibits global cellular protein synthesis. Despite growing evidence suggesting the involvement of PKR in bacterial infections, little is known about its expression, regulation and cellular role in nonviral infections. The aim of this work was to determine the expression and regulation of PKR in response to stimulation of human THP-1 monocytes with bacterial agonists of TLR2/4. Treatment of cells with Pam3CSK4 or lipopolyssacharide (LPS) resulted in an increase in PKR mRNA and protein levels. Robust PKR expression at later times correlated with a decrease in global protein synthesis. PKR was also required to regulate the inhibition of protein synthesis triggered by LPS in mouse splenocytes. Surprisingly, no increase of IFN-beta or IFN-alpha mRNA levels was detected after treatment of THP-1 cells with toll-like receptor (TLR) agonists. In accordance with this, the supernatants from LPS or Pam3CSK4-treated cells lacked the ability to activate the PKR and ISG56 promoters in gene reporter assays carried out in HEK293T cells. The expression of PKR induced by TLRs agonists was dramatically impaired when cells were treated in the presence of tosyl-phenylalanyl chloromethylketone or Mithramycin, suggesting that NF-kappaB and Sp1 transcription factors, but not those activated by IFNs, regulate the expression of PKR in human monocytes.

10 Replication of dsRNA virus - YouTube




The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow.

The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:

From which DNA strand is the mRNA synthesized? - …

N2 - Small circular double-stranded RNAs (dsRNAs) were synthesized. The structural analysis by atomic force microscopy gave direct images for the interpretation of the structural strain present in circular dsRNAs. Finally, we demonstrated that circular dsRNA caused RNAi effect in cells.