RNA hybrid to double-stranded cDNA (dscDNA ..

This one step protocol facilitates the synthesis of single stranded cDNA from mRNA.

A Cost-effective Double-Stranded cDNA Synthesis for …

PCR (Polymerase Chain Reaction) is a revolutionary method developed by Kary B Mullis (awarded Nobel Prize for chemistry in 1993) in the 1983. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the template strand of DNA. This technique is having an impact on many areas of , genetics, recombinant DNA research molecular biology, forensic analysis, , and medical diagnostics.

Incubate at 14°C for 12 hours and recover double-stranded cDNA by GENECLEAN II.

the double stranded cDNA is synthesized from single stranded ..

If the RNA template is not degraded after first strand cDNA synthesis, it can bind to the newly synthesized cDNA and restrict the accessibility of primers during subsequent PCR amplification. RNase H-mediated destruction of the template can prevent this problem and improve the sensitivity of RT-PCR analysis. However, an additional RNase H incubation step prolongs the reaction time and incurs additional costs for the often expensive RNase H. The Roche Transcriptor Reverse Transcriptase has endogenous RNase H activity ideal for digesting the original RNA template.

Using the RevertAid™ First Strand cDNA Synthesis Kit Quantify –We.2 MonsterScript™ 1st-Strand cDNA Synthesis Kit 1.

A fraction of the first strand cDNA product was used to amplify sequences specific for three different messenger RNAs using 1X LongAmp™ Taq 2X Master Mix ( ).

14/03/2012 · A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays


facilitates the synthesis of single stranded cDNA ..

Mint-2 cDNA synthesis kit is designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly(A)+ RNA. Synthesized cDNA can be used for construction of cDNA libraries, subtractive hybridization (SSH), high-throughput sequencing on the next generation sequencing platforms (Roche/454, ABI/SOLiD or Illumina/Solexa), and other applications.

for the synthesis of blunt-ended double-stranded cDNA

SYBR Green I is a double-stranded DNA specific dye. During each phase of DNA synthesis, SYBR Green I dye which is included in the reaction mix, binds to the amplified PCR products. Resulting amplicons can then be detected by their fluorescence.
Hot start protocols used with the FastStart Essential DNA Green Master have been shown to significantly improve specificity, sensitivity, and PCR yield. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. This means that there is no elongation during the period when primers can nonspecifically bind. FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (., a pre-incubation step at +95°C for 10 minutes).

The FastStart Essential DNA Green Master can be used in conjunction with heat-labile for carryover prevention during PCR. It is ideal for two-step RT-PCR applications, for example, when used downstream of cDNA synthesis using.

and Double-Stranded cDNA Synthesis 3.

SYBR Green I is a double-stranded DNA specific dye. During each phase of DNA synthesis, SYBR Green I dye which is included in the reaction mix, binds to the amplified PCR products. Resulting amplicons can then be detected by their fluorescence.
Hot start protocols used with the FastStart Essential DNA Green Master have been shown to significantly improve specificity, sensitivity, and PCR yield. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. This means that there is no elongation during the period when primers can nonspecifically bind. FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (., a pre-incubation step at +95°C for 10 minutes).

The FastStart Essential DNA Green Master can be used in conjunction with heat-labile for carryover prevention during PCR. It is ideal for two-step RT-PCR applications, for example, when used downstream of cDNA synthesis using.

5 μl of the double-stranded cDNA, ..

High mutation rate is a hallmark of retrovirus replication. This originates in the mechanism of genome replication by the viral-encoded reverse transcriptase, which converts the genomic RNA of the virus to double-stranded DNA (dsDNA). During this process, reverse transcription produces frequent replication errors. One accepted explanation of this inaccuracy is the lack of RT 3´-5´ exonuclease activity. The naturally high error rate of reverse transcriptases is not optimal for many applications.