DNA, RNA and Protein Synthesis - Modesto Junior …

Protein was extracted from  using the Presto™ DNA/RNA/Protein Extraction Kit.

Ward's® DNA Structure And Protein Synthesis Kit | VWR

Available now! With all 3 kits (DNA/RNA, Protein, and tRNA), students will be able to complete protein synthesis, transcribing a gene into mRNA, translating the mRNA into a protein chain, and folding the protein chain into a functional shape.

LA72 DNA, RNA Protein Synthesis - Science Lab Supplies

DNA transcription is the process of making a single strand complementary RNA copy of DNA. Data is copied from the DNA to the RNA with the aid of the enzyme RNA polymerase. Using this process, the genetic information stored in the DNA is carried in the form of RNA to other parts of the cell. In eukaryotic cells a gene begins with a promoter region and an initiation code and ends with a termination code. However, the intervening gene sequence contains patches of nucleotides that have no meaning. If they were used in protein synthesis, the resulting proteins would be worthless. Eukaryotic cells prune these segments from the mRNA after transcription. RNA polmerase synthesizes a strand of pre-mRNA that initially includes copies of the meaningful mRNA coding sequences (exons) and the meaningless mRNA coding sequences (introns). Soon after its manufacture, this pre-mRNA molecule has the meaningless introns clipped out and the exons spliced together in the final version of mature mRNA

Your students can build a model of DNA with this simple and colorful system. The parts are color-coded to represent the purines, pyrimidines, deoxyribose and phosphodiester groups that make up the double helix of DNA. This kit includes differently sized purines and pyrimidines, the correct number of hydrogen bonds and the minor and major grooves are shown. Ideal for modelling DNA replication. Use together with the RNA Protein Synthesis Kit to model translation and transcription.


You will learn the structure and function of DNA ..

1. Along with your unit test on the DNA unit, include the "practical" test supplement, requiring students to decipher a short DNA message during a test. Provide the (including tRNA Dictinary), the special answer sheet, and a unique word for each student (on little slips of paper, which you can prepare from the sample sentences provided, or use the 33 3-letter provided (along with a for you to use in grading the test.).. Insist that each step be shown and the molecules involved be labeled (DNA, mRNA, tRNA, "protein" (as the "meaningful amino acid sequence"). Include completeness and accuracy in showing these steps and labels in your grading.

Lab-Aids™ DNA-, RNA-Protein Synthesis Modeling Kit

Long strands of DNA along with proteins
called histones are tightly wound together to form chromosomes.
In order to synthesize a protein, information must be transferred from the DNA in the nucleus of
the cell to the ribosomes in the cytoplasm of the cell.

Phage DNA Isolation Kit - Bio-Synthesis Inc

Translation is a process where the code for making a protein (found on a molecule of
RNA) is translated into specific amino acids that will comprise apolypeptide (protein).
In this lab, students will simulate the process of protein synthesis by transcribing DNA molecules
into RNA molecules and then subsequently, translating RNA molecules into amino acids.
Students will also extract DNA from an onion.
Resources and Assignments
Multimedia Resources

None

Required Assignments

Lesson 10 Lab 8

Required Materials

From the Lab Kit


DNA template cards



Universal genetic code reference card



Ribosome circle



Thermometer



Funnel



100 ml graduated cylinder



2 coffee filters



Measuring spoon (teaspoon)



2 teaspoons salt

Student Provided


Pen or pencil



Standard printer paper (1 sheet)



Scissors



Onion



1 small pot



120 ml water



2 tablespoons liquid dish detergent



Fork (Needed only if a blender or food processor is not available)



Knife



Cutting board or other surface for cutting the onion



Additional water for heating (3-4 cups tap water)



2 small glass, ceramic, or metal mixing bowls



2 cups ice or access to a freezer



2 clear drinking glasses (12-16 oz)



100 ml Isopropyl alcohol (rubbing alcohol), 71% or higher



Blender or food processor



Tape

Optional: (if the student wants to preserve the extracted DNA)



Alcohol (100-200 ml)



Focusing Your Learning

Small clear glass container with a lid

Wooden skewer or toothpick

Lab Objectives
By the end of this lesson, you should be able to:
1.