H., His6 tag-assisted chemical protein synthesis.
1961, 83, 3080) made the first total synthesis of arachidonic acid.
Imai J demonstrated that the oxidative desaturation of a saturated fatty acid (palmitic acid) is depressed in diabetic rats ().
Kennedy EP described the general pathways of the glycerolipid biosynthesis in animal cells ().
Bremer J et al.
biochemistry : ’’de novo’’ synthesis of a complex molecule, ..
Ribonucleic acid, RNA (French : acide ribonucléique, ARN) A chemical found in the nucleus and cytoplasm of cells; it plays an important role in protein synthesis and other chemical activities of the cell. The structure of RNA is similar to that of DNA. There are several classes of RNA molecules, including messenger RNA, transfer RNA, ribosomal RNA, and other small RNAs, each serving a different purpose.
(French : traduction) The process of forming a specific protein having its own amino acid sequence determined by the codons of the messenger RNA. The ribosome and transfer RNA are necessary for this process.
De novo synthesis and development of an RNA enzyme
FSH plays crucial roles in differentiation of granulosa cells and development of follicles. Considering the broad scope of FSH effects, a large number of genes are likely responsive to the hormone. However, only a limited number of genes have been identified as FSH-regulated genes, particularly during the preantral stage. In an attempt to better define genes involved in follicular development, we examined primary granulosa cell cultures, an undifferentiated rat ovarian granulosa cell line and rat ovaries, using differential display, quantitative RT-PCR, Northern blot analysis, and in situ hybridization. We report, for the first time, that nicotinamide adenine dinucleotide phosphate-dependent cytosolic T3-binding protein mRNA is expressed in the ovary, particularly in the granulosa cell layer of preantral and early antral follicles, but not in large preovulatory follicles. Its expression markedly declines in response to FSH, which is dependent on the period of the exposure. This FSH-responsive down-regulation is dependent on granulosa cell differentiation and follicular development. FSH down-regulates the mRNA via the adenylyl cyclase/cAMP pathway, and the down-regulation requires de novo synthesis of a regulatory protein(s). The cytosolic T3-binding protein may play a significant role in the regulation of steroidogenesis and follicular development in the mammalian ovary.
De Novo Purine Synthesis; De novo review;
Understanding how cells orchestrate molecular events leading to de novo Nrf2 protein translation under oxidative stress is important for dialing up this pathway for organ protection.
ATG: synthetic genes, de novo DNA synthesis and more..
In general usage, de novo is a Latin expressionmeaning "from the beginning," "afresh," "anew," "beginning again."It is used in:
De novo pathway - Biology-Online Dictionary
Amino acids contribute to carbohydrate synthesis via gluconeogenesis, to fat synthesis or energy production via acetyl-CoA, and special nitrogen compounds such as catecholamines (neurotransmitters), thyroid hormones, creatine(-phosphate), the protoporphyrin ring (heme), and contribute to nucleic acid and phospholipid synthesis as nitrogen group donor.