DNA Synthesis is Semi-discontinuous - Biology LibreTexts

DNA Synthesis is Semi-discontinuous

of synthesis, use of RNA primer, use of dNTPs, and continuous vs

Discontinuous innovation is characterized by lateral or divergent thinking, by looking outside of defined boundaries, and by discovery of new knowledge related to both market need and technological capability.

Continuous vs discontinuous--leading and lagging strands ..

When visited on 16 November 2003, Howell found the summit at ~ 32 m below sea level, a significant change from the island seen three months earlier.In summary, table 2 shows discontinuous observations of elevation/depth of the summit of Kavachi over a period of about 3.5 years (2000-2003). A compilation listing the stated elevations (+) and depths (-) of Kavachi's summit over a 3.5-year period.

Assay methods are given in the instruction manuals supplied with peptide synthesis kits, and with peptide sets. Software which assists with reading and plotting the results of the assays is also supplied. Practical guides are available in the Pinpoints series, including the following titles: "Using Antigen Competition to Identify Sequential (Linear) Epitopes" and "ELISA Method Using Biotinylated Peptides". Conclusions Epitope mapping with overlapping synthetic peptides is the most efficient way to identify the linear antigenic determinants defined by a particular serum or monoclonal antibody. A large proportion of the total epitope "universe", the epitopes which are assembled or discontinuous, are not accessible by this method. However, linear epitopes are amenable to detailed investigation, including establishing the contribution of each residue to the overall binding requirements of the epitope. The relevance of peptide epitopes detected with polyclonal antibodies should be checked using a secondary technique such as competition with native antigen; or elution of antibody from the binding peptide, followed by a test of the eluted antibody in a supplementary immunoassay.
Epitope mapping with synthetic peptides is ideally done with pin-bound peptide (for high sensitivity) and with solution- phase peptide. Alternatively, to scan only for the high affinity or high concentration antibodies, use biotinylated solution-phase peptides alone.


02/04/2007 · Different Kinds of Reactions

OT is generally about theemergenceof a new belief system and necessarily involves reframing, which is adiscontinuous change in the organization's or group's shared meaning orculture.

Discontinuous reactions are always net-transfer reactions

OT is defined as, "Profound,fundamentalchanges in thought and actions, which create an irreversiblediscontinuity inthe experience of a system" (Adams 1984).

Continuous reactions involve phases that may vary in composition.

Replication occurs bi-directionally from the origin of replication (initiation site). DNA polymerase can only add deoxyribonucleotide residues in a 5' to 3' direction. Therefore, as replication proceeds in a given direction from the origin, one of the daughter strands (leading) is synthesized continuously. The other daughter strand (lagging) is synthesized discontinuously as short segments of DNA that are called Okazaki fragments (Fig. 3, right). The Okazaki fragments are later joined to form a continuous DNA strand.

discontinuous replication definition - Northwestern …

His interest go from microstructure evolutions in metallic alloys ( precipitation, phase transformation, dislocation structures and stability), to relations between microstructures and mechanical properties (Plasticity and Fracture). In these classical topics of physical metallurgy, he has made important contributions to the modelling of precipitation in aluminium alloys , on the morphological stability of precipitates in Ni based super alloys, on the austenite to ferrite transformation kinetics, on discontinuous precipitation and DIGM, and on multilayers reactions. He has also contributed to the statistical analysis of plastic instabilities, to the theory of work hardening of metals with small grain sizes, or with precipitates, on the development of constitutive equations for creep of industrial alloys, on the nucleation of recrystalliation and on the coupling of recrystallisation, recovery and precipitation. He has also a specific interest for nuclear materials , involving the dynamics of irradiation defects, irradiation hardening and embrittlement, irradiation creep. He has also studied specific effects of coupled mechanical loading and corrosion in nuclear materials.