of CO2 concentration on the rate of photosynthesis of the ..
The ratio of chlorophyll to organic content of the cells varies with the physiological state of the cells and the ration of carotenoid to chlorophyll can give a useful index of, for example, nitrogen deficiency.Determination of pigment content of phytoplanktonFilter a known volume (250ml–1litre) of well shaken water through a glass-fibre filter under vacuum.
PHOTOSYNTHESIS RATE, CHLOROPHYLL CONTENT AND INITIAL ..
6CO + 6H O ® C H O + 12O (in the presence of light energy and chlorophyll) Aim- The aim of the experiment is to determine what effect light intensity has upon the rate of photosynthesis of Canadian Pondweed (Elodea)....
Cross species interpretation and comparison of the SPAD-502 Chlorophyll Index (SCI) are not advisable as the readings generated by SPAD-502 meter are affected by leaf structure. Re-establishing the SCI data into expressive readings such as actual chlorophyll concentration is necessary for results interpretation. Oil palm leaf nitrogen concentration, chlorophyll concentration and photosynthetic rate were highly correlated with R2 of above 0.80. With proper calibration equation, the SPAD-502 chlorophyll meter are extremely versatile for rapid determination of oil palm leaf nitrogen and chlorophyll concentration as well as photosynthetic rate. Correlation between leaf potassium concentration and SCI were poor as potassium is not the constituent of the photosynthetic pigments. Hence the usage of SPAD-502 meter for rapid in-field determination of potassium concentration is not recommended.
To investigate the effect of carbon dioxide concentration on the ..
A limiting factor is a factor that controls a process. Light intensity, temperature and carbon dioxide concentration are all factors which can control the rate of photosynthesis. Usually, only one of these factors will be the limiting factor in a plant at a certain time. This is the factor which is the furthest from its optimum level at a particular point in time. If we change the limiting factor the rate of photosynthesis will change but changes to the other factors will have no effect on the rate. If the levels of the limiting factor increase so that this factor is no longer the furthest from its optimum level, the limiting factor will change to the factor which is at that point in time, the furthest from its optimum level. For example, at night the limiting factor is likely to be the light intensity as this will be the furthest from its optimum level. During the day, the limiting factor is likely to switch to the temperature or the carbon dioxide concentration as the light intensity increases.
The light energy is absorbed by chlorophyll and ..
So how can these factors have an effect on the rate of photosynthesis? Lets start off with the light intensity. When the light intensity is poor, there is a shortage of ATP and NADPH, as these are products from the light dependent reactions. Without these products the light independent reactions can't occur as glycerate 3-phosphate cannot be reduced. Therefore a shortage of these products will limit the rate of photosynthesis. When the carbon dioxide concentration is low, the amount of glycerate 3-phosphate produced is limited as carbon dioxide is needed for its production and therefore the rate of photosynthesis is affected. Finally, many enzymes are involved during the process of photosynthesis. At low temperatures these enzymes work slower. At high temperatures the enzymes no longer work effectively. This affects the rate of the reactions in the Calvin cycle and therefore the rate of photosynthesis will be affected.
Photosynthesis Respiration and Plant Growth
As we can see, there is a close relationship between the action spectrum and absorption spectrum of photosynthesis. There are many different types of photosynthetic pigments which will absorb light best at different wavelengths. However the most abundant photosynthetic pigment in plants is chlorophyll and therefore the rate of photosynthesis will be the greatest at wavelengths of light best absorbed by chlorophyll (400nm-525nm corresponding to violet-blue light). Very little light is absorbed by chlorophyll at wavelengths of light between 525nm and 625 (green-yellow light) so the rate of photosynthesis will be the least within this range. However, there are other pigments that are able to absorb green-yellow light such as carotene. Even though these are present in small amounts they allow a low rate of photosynthesis to occur at wavelengths of light that chlorophyll cannot absorb.
Rate of Photosynthesis :: Papers - 123HelpMe
Relationship between SCI with photosynthetic activity: Photosynthetic rate of oil palm leaf correlated positively to SCI following a logarithmic model with R2 of 0.82 (). Photosynthetic rate are affected by the chlorophyll concentration. As the chlorophyll concentration correlates well to SCI, hence the photosynthetic rate can be expected to correlate well with SCI. Good correlation between photosynthesis rate and SCI increases the robustness of SPAD-502 meter for non-destructive and rapid determination of photosynthetic rate in the field, thus minimizing the need of bulky equipment such as the photosynthetic meter.