RNA oligonucleotide synthesis via 5′‐silyl‐2′‐orthoester chemistry.

Chemical synthesis of a biologically active natural tRNA with its minor bases.

Synthesis of chemically modified DNA

Okada N, Noguchi S, Kasai H et al. (1979) Novel mechanism of post‐transcriptional modification of tRNA. Insertion of bases of Q precursors into tRNA by a specific tRNA transglycosylase reaction. Journal of Biological Chemistry 254: 3067–3073.

A base‐labile group for 2′‐OH protection of ribonucleosides: A major challenge for RNA synthesis.

Chemical synthesis of RNA (including RNA with unusual constituents).

Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite. Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. Oligonucleotides containing GalNAc can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. Glen Research offers these GalNAc C3 products under an agreement with AM Chemicals LLC.

Recent advances in the high‐speed solid phase synthesis of RNA.

Brulé H, Elliott M, Redlak M, Zehner ZE and Holmes WM (2004) Isolation and characterization of the human tRNA‐(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein. Biochemistry 43: 9243–9255.

On the application of t‐butyldimethylsilyl group in chemical RNA synthesis.


Oligo‐ and poly‐nucleotides: 50 years of chemical synthesis.

Kammen HO, Marvel CC, Hardy L and Penhoet EE (1988) Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I. Journal of Biological Chemistry 263: 2255–2263.

Removal of t‐butyldimethylsilyl protection in RNA‐synthesis.

Lauhon CT, Erwin WM and Ton GN (2004) Substrate specificity for 4‐thiouridine modification in Escherichia coli. Journal of Biological Chemistry 279: 23022–23029.

The chemical stability of abasic RNA compared to abasic DNA

Hamdane D, Argentini M, Cornu D, Golinelli‐Pimpaneau B and Fontecave M (2012) FAD/folate‐dependent tRNA methyltransferase: flavin as a new methyl‐transfer agent. Journal of the American Chemical Society 134: 19739–19745.

Synthesis of chemically modified DNA

Lee SR and Collins K (2005) Starvation‐induced cleavage of the tRNA anticodon loop in Tetrahymena thermophila. Journal of Biological Chemistry 280: 42744–42749.

Chemical synthesis of branched RNA. - National Center …

Chemical synthesis of a very long oligoribonucleotide with 2‐cyanomethoxymethyl (CEM) as the 2′‐O‐protecting group: structural identification and biological activity of a synthetic 110mer precursor‐microRNA candidate.

Recent Advances in the Chemical Synthesis of RNA - …

Gu X, Ivanetic KM and Santi DV (1996) Recognition of the T‐arm of tRNA by tRNA (m5U54)‐methyltransferase is not sequence specific. Biochemistry 35: 11652–11659.

an Buy Adenine | DNA/RNA Synthesis chemical | Price | …

McCraith SM and Phizicky EM (1991) An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2′‐phosphate from ligated tRNA to an acceptor molecule. Journal of Biological Chemistry 266: 11986–11992.