Cell proliferation, cell death, and differentiation in gliomas

In this thesis I investigate the developmental and cellular consequences of mutationsin ORC1.

T Cell Proliferation Assays Research

Although all three types may be proliferated and present a serious threat to international security, the focus is placed on nuclear weapons because of their enormous destructive capacity.

The low currents decreased and higher currents increased the cell-substrate adhesion forces.

Mathematical modelling of cell migration and …

The electric stimulation was applied to two different types of mammalian cells, mouse myoblasts and adipose-derived stem cells that were either in a direct contact with the electrodes or in a contact with the electrodes through the electrolyte.

The applied electric current changed the cell spreading characteristics on the electrode, and induced the more elongated cell morphology even when the cells were not cultured directly on the electrode.

However, after a certain threshold, the increase in current dose resulted in decrease in the cell viability and sometimes also on the cell proliferation rates.

engineering for improving cell proliferation and ..

(2010) State Diagram for Contact-Inhibition of Proliferation: A Quantitative Framework for Modulating Growth Patterns in Epithelial Cell Clusters. Dissertation (Ph.D.), California Institute of Technology.

Regulation of T-cell proliferation and B-CLL apoptosis …

Stimulation of the stem cells with electric current and electrochemically released Cu2+ induced the upregulation of neuron-specific genes and proteins, whereas stimulation with current only mainly induced changes in the cell morphology.

As demonstrated in this thesis, electric stimulation induces changes in many cellular functions and might offer an easy and cost-effective method to regulate them in future in vitro and in vivo applications.

Markers expression of cell proliferation and apoptosis ..

Cell-cell contacts play a key role in the assembly and integrity of epithelial tissues. Cell-cell contact is not only a mere physical link between neighboring cells, but also a critical regulator of many cell behaviors including proliferation. Contact-inhibition of proliferation is a hallmark of normal epithelial tissues. Cancer development involves the loss of this key constraint. Both biochemical and physical mechanisms mediating contact-inhibition are emerging. A current, principal challenge is elucidating how the integrated performance of these mechanisms enforce or modulate contact-inhibition in a rich microenvironment that includes multiple, potentially conflicting cues such as soluble growth factors (GFs) and extracellular matrix (ECM). Here, we propose a quantitative paradigm for contact-inhibition of proliferation. Our quantitative analysis of single cells within multicellular aggregates reveals that epithelial cells transition from a contact-inhibited to contact-independent mode of proliferation at a critical threshold EGF level. This transition point is a tunable property and can be modulated by varying the level of cell-cell contact. Furthermore, the proximity to this transition point is a quantitative gauge of “degree” of contact-inhibition. Using this metric, we demonstrate that stiffening the adhesive matrix, a widely observed phenomenon during cancer development, leads to the quantitative, progressive reduction in the EGF threshold needed to induce contact-independent proliferation. Thus, stiffening the ECM moves an epithelial cell system closer to the transition to contact-independence, thereby quantitatively reducing the amount of EGF amplification needed to induce population-wide proliferation. Our results reveal that the potent effect of substratum compliance on contact-inhibition involves changes in contact-maturation and multicellular mechanics. The proposed quantitative model of contact-inhibition provides fundamental insights into our understanding of tissue morphogenesis and cancer progression in multicellular organisms. Furthermore, our findings provide design principles for engineering multicellular growth in applications such as tissue engineering.


While the cooperative importance of this treaty cannot be understated, it is not the only International Institution that has a prominent place in the non-proliferation, disarmament and nuclear safety realm.