Biocatalytic synthesis of (S ..

.The scientist who researched and applied the new biocatalytic synthesis of adipic acid ..

Biocatalytic asymmetric synthesis of ..

Other scientists promoted the biocatalytic method of synthetic adipic acid from D-glucose. It is achieved with genetically transgenic bacteria Klebsiella pneumoniae, a non-toxic strain of Εscherichia coli, (Enterobactiriaceae).The scientist who researched and applied the new biocatalytic synthesis of adipic acid was awarded the “Presidential Green Chemistry Challenge Awards Program” in 1998 in the USA.

Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA.

hispanica resulted in a total termination of PHA synthesis

Polycaprolactone (Figure 2c) is a synthetic polymer prepared by ring opening polymerisation of caprolactone. The mechanical properties are similar to polyolefins. It is similar to PHAs and fully biodegradable, but degrades at a lower rate compared to PHAs. Due to lower melting temperature of about 60°C, the polymer is mainly used in polymer blend or as a matrix for biodegradable composites.

Use of the two-liquid phase concept to exploit kinetically controlled multistep biocatalysis.
Biotechnology and Bioengineering, 81(6):683-694, PubMed

Phenol-hypochlorite reaction for determination of …

Cyanobacteria form a group of photosynthetic nitrogen-fixing bacteria, which are present in almost all aquatic or terrestrial habitats, and are increasingly in the spotlight as third generation PHA producers [69,70]. Shrivastav and colleagues [71] studied the marine photoautotroph cyanobacterium Spirulina subsalsa, originally described as an isolated from samples from the Indian coast. This organism turned out to display enhanced PHA production when exposed to elevated salinity levels.

Discovery of N-(2-Chloro-6-methyl- phenyl)-2-(6-(4 …

Danis et al. [68] carried out similar experiments with five extremely halophilic archaeal isolates in order to trace the strain with the highest PHA-production capacity. PHA production of each isolate was individually examined on various inexpensive carbon sources such, e.g., sucrose, whey, corn starch, and waste of apples, melons and tomatoes. Among these feedstocks, corn starch turned out to be a promising substrate for PHA biosynthesis. Among the investigated strains, isolate 1KYS1 displayed the highest PHA biosynthesis capacity. Comparative 16S rRNA gene sequence analysis revealed the close relationship of 1KYS1 to the genus Natrinema, especially to the species Natrinema pallidum JCM 8980. On starch as sole carbon substrate, 1KYS1 reached a PHA content in CDM of about 53.14 wt.-%. Large and uniform intracellular PHA granules were observed by transmission electron microscopy (TEM), and identified as the copolyester PHBHV. PHBHV produced by 1KYS1 was blended with poly (ethylene glycol)l of low molar mass for preparation of biocompatible films which can be used for drug release experiment with Rifampicin as model compound. Most efficient drug delivery was achieved at 37 °C and a pH-value of 7.4.

10.1021/jm049486a - American Chemical Society

Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis.
Advanced Synthesis and Catalysis, 353:3485 – 3495