What is ATP Synthesis? | Yahoo Answers
The mechanism that drives ATP synthesis seems to depend upon a binding charge conception in which catalytic sites on the β-subunits have different affinities for nucleotides and are designated , , and . .These are coloured , , and respectively. The sites bind the substrates (ADP and phosphate)
ATP Synthase- enzyme that catalyses ATP synthesis and hydrolysis ..
The easiest experimental system for ATP synthesis measurements are native photosynthetic membranes (derived from plant chloroplasts or phototrophic bacteria): they let you control by simply turning on and off the light. Moreover, the value at saturating illumination isusualy very high in photosynthetic membranes. So by adjusting the power of illumination one can finely and precisely adjust . Moreover, in many cases the intrinsic carotenoid pigments present innative photosynthetic membranes serve as a voltmeter: their absorption spectra shift inresponse to electric field across the membrane. So if no Delta_pH is present, is composed of transmembrane membrane voltage only and can be directly monitored optically.
This method works for ATP synthase and for recombinant PS3 thermophilic enzymeexpressed in strains lacking its own ATP synthase. I do not see any reason why it would not work for other recombinant ATP synthases.
is generated by respiratory chain enzymes: NADH or succinate are good substrates for that. ATPsynthesis can be detected by luciferin-luciferase.