Second-Strand cDNA Synthesis Kit-dNTP based

Order Second-Strand cDNA Synthesis Kit-dNTP/dUTP based (ABIN4219228) directly from us.
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Roche transcriptor cDNA synthesis kit (1st strand).

noyara on Wed Sep 14 15:33:36 2011 said:

Hi Noyara,
My PCR worked after i did second strand cDNA synthesis (using Klenow). I am yet to sequence it but i got product of expected size. I didn't use any kit. I used phusion high fidelity dna polymerase from NEB. Roche transcriptor cDNA synthesis kit (1st strand). T4 ligase from NEB.

You can choose any product. I am happy with Phusion enzyme, i could able to amplify 4.5 kb gene.

Yes i want to express this in cell lines.


PrimeScript First Strand cDNA Synthesis Kit - Separations
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cDNA Synthesis Kits | Biocompare Editorial Article

Using the supplied Oligo ([dT24] T7 promoter)primer, cDNA is synthesized containing a T7 RNA polymerasepromoter for the subsequent transcription reactionwith T7 RNA polymerase.

Microarray Target Purification Kit

The Microarray Target Purification Kit is specificallydesigned for the purification of a variety of synthesizednucleic acids in the target labeling and amplificationprocess, such as: The kit will remove residual nucleotides, primers, proteins,nucleases and other reaction components whichwill otherwise interfere in the workflow for highlysensitive microarray hybridization.

The PrimeScript First Strand cDNA Synthesis Kit is ideal for reverse ..
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Viva 2-step RT-PCR kit is specially designed to provide reliable synthesis of full-length cDNA and convenient application of cDNA in PCR. M-MuLV Reverse Transcriptase synthesize complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as RNA strand does not degraded in DNA-RNA hybrid during first strand cDNA synthesis. With variety of kit options for standard PCR and long PCR, Viva 2-step RT-PCR kit provides flexibility in an easy use format.

27/11/2009 · An editorial article discussing cDNA Synthesis Kits
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